{"id":571,"date":"2015-06-10T15:29:33","date_gmt":"2015-06-10T15:29:33","guid":{"rendered":"http:\/\/ri.itservices.manchester.ac.uk\/icsf\/?page_id=571"},"modified":"2015-06-10T15:29:33","modified_gmt":"2015-06-10T15:29:33","slug":"cufflinks","status":"publish","type":"page","link":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/software\/applications\/cufflinks\/","title":{"rendered":"Cufflinks"},"content":{"rendered":"<h2>Overview<\/h2>\n<p>Cufflinks assembles transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples. It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one, taking into account biases in library preparation protocols.<\/p>\n<p>Version 2.2.1 and 2.1.1 binaries are available on the iCSF.<\/p>\n<h2>Restrictions on use<\/h2>\n<p>All users may access and use Cufflinks. The software is open source. Users should consult the <a href=\"http:\/\/opensource.org\/licenses\/BSL-1.0\">Boost Software License 1.0<\/a> for further information. <\/p>\n<h2>Set up procedure<\/h2>\n<p>To access the software load <em>one<\/em> of the following modulefiles for the version you require:<\/p>\n<pre>\r\nmodule load apps\/binapps\/cufflinks\/2.2.1\r\nmodule load apps\/binapps\/cufflinks\/2.1.1\r\n<\/pre>\n<h2>Running the application<\/h2>\n<p>It may be necessary to use other applications in conjunction with Cufflinks. <\/p>\n<p>Panagiotis Papastamoulis, from FLS has very kindly provided some <a href=\"http:\/\/ri.itservices.manchester.ac.uk\/csf-apps\/wp-content\/uploads\/cufflinksExample.pdf\">documentaion illustrating how to use Cufflinks on the CSF<\/a>, (note <em>CSF<\/em>, not iCSF<\/em>) in conjunction with other tools and including example jobscripts. On the iCSF you don&#8217;t need to use jobscripts &#8211; you can simply run the commands specified in the jobscripts directly on the iCSF node you have been logged in to.<\/p>\n<h2>Troubleshooting<\/h2>\n<p>You may see the following error when using cufflinks, but it is nothing to worry about and the software will still run.<\/p>\n<pre>cufflinks: \/lib64\/libz.so.1: no version information available (required by cufflinks)<\/pre>\n<h2>Further info<\/h2>\n<ul>\n<li>The <a href=\"http:\/\/cufflinks.cbcb.umd.edu\/index.html\">Cufflinks website<\/a> has a manual, FAQ and index and annotations downloads.<\/li>\n<li>Related applications available on the iCSF: <\/li>\n<ul>\n<li><a href=\"\/icsf\/software\/applications\/bowtie\">Bowtie<\/a><\/li>\n<li><a href=\"\/icsf\/software\/applications\/bowtie2\">Bowtie2<\/a><\/li>\n<li><a href=\"\/icsf\/software\/applications\/tophat\">Tophat<\/a><\/li>\n<li><a href=\"\/icsf\/software\/applications\/samtools\">Samtools<\/a><\/li>\n<\/ul>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Overview Cufflinks assembles transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples. It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Cufflinks then estimates the relative abundances of these transcripts based on how many reads support each one, taking into account biases in library preparation protocols. Version 2.2.1 and 2.1.1 binaries are available on the iCSF. Restrictions on use All users may access and.. <a href=\"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/software\/applications\/cufflinks\/\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":43,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-571","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/pages\/571","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/comments?post=571"}],"version-history":[{"count":1,"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/pages\/571\/revisions"}],"predecessor-version":[{"id":572,"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/pages\/571\/revisions\/572"}],"up":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/pages\/43"}],"wp:attachment":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/icsf\/wp-json\/wp\/v2\/media?parent=571"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}