{"id":984,"date":"2018-11-23T12:35:47","date_gmt":"2018-11-23T12:35:47","guid":{"rendered":"http:\/\/ri.itservices.manchester.ac.uk\/csf3\/?page_id=984"},"modified":"2018-11-28T17:21:21","modified_gmt":"2018-11-28T17:21:21","slug":"homer","status":"publish","type":"page","link":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/software\/applications\/homer\/","title":{"rendered":"HOMER"},"content":{"rendered":"<h2>Overview<\/h2>\n<p>HOMER (Hypergeometric Optimization of Motif EnRichment) is a suite of tools for Motif Discovery and next-gen sequencing analysis.<\/p>\n<p>Version 4.10.3 is installed on the CSF:<\/p>\n<ul>\n<li>The base software is installed, datasets mouse-o, mouse-p, mm8, hg19, hg38 have been included.<\/li>\n<li>Weblogo is no longer required so is NOT installed.<\/li>\n<li>BLAT has NOT been included.<\/li>\n<\/ul>\n<h2>Restrictions on use<\/h2>\n<p>HOMER is free software and available to all users of the system. Please ensure you cite your usage in any results and publications as per the <a href=\"http:\/\/homer.salk.edu\/homer\/index.html\">HOMER website<\/a>.<\/p>\n<h2>Set up procedure<\/h2>\n<p>To access the software you must first load the modulefile:<\/p>\n<pre>\r\nmodule load apps\/binapps\/homer\/4.10.3\r\n<\/pre>\n<p>We recommend that you do this in your jobscript (rather than on the command line before submission as per CSF2).<\/p>\n<h2>Running the application<\/h2>\n<p>Please do not run HOMER on the login node. Jobs should be submitted to the compute nodes via batch.<\/p>\n<p>You <em>may<\/em> run the tool on the login node with the <code>-help<\/code> flag to see the command-line flags accepted by the tool.<\/p>\n<p>Note that if the HOMER tool you run generates parsed files (e.g., ) then you should inform the tool where to write those files. By default it may try to write them in the centrall installation area to which you do not have write access. Check whether your tool accepts the flag:<\/p>\n<pre>\r\n-preparsedDir <em>path\/to\/local\/folder<\/em>\r\n<\/pre>\n<p>which allows you to specify the name of a local folder (e.g., <code>~\/scratch\/homer_data<\/code>).<\/p>\n<h3>Serial batch job submission<\/h3>\n<p>Create a jobscript, for example:<\/p>\n<pre>\r\n#!\/bin\/bash --login\r\n#$ -cwd             # Job will run from the current directory\r\n\r\n# Load the software\r\nmodule load apps\/binapps\/homer\/4.10.3\r\n\r\n# Example using the findMotifsGenome.pl tool:\r\nfindMotifsGenome.pl peaks.txt mm8r peakAnalysis -size 200 -len 8 -preparsedDir ~\/scratch\/my_homer_data\r\n<\/pre>\n<p>Submit the jobscript using: <\/p>\n<pre>qsub <em>scriptname<\/em><\/pre>\n<p>where <em>scriptname<\/em> is the name of your jobscript.<\/p>\n<h2>Further info<\/h2>\n<ul>\n<li><a href=\"http:\/\/homer.salk.edu\/homer\/index.html\">HOMER website<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Overview HOMER (Hypergeometric Optimization of Motif EnRichment) is a suite of tools for Motif Discovery and next-gen sequencing analysis. Version 4.10.3 is installed on the CSF: The base software is installed, datasets mouse-o, mouse-p, mm8, hg19, hg38 have been included. Weblogo is no longer required so is NOT installed. BLAT has NOT been included. Restrictions on use HOMER is free software and available to all users of the system. Please ensure you cite your usage.. <a href=\"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/software\/applications\/homer\/\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":86,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-984","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/pages\/984","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/comments?post=984"}],"version-history":[{"count":8,"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/pages\/984\/revisions"}],"predecessor-version":[{"id":1200,"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/pages\/984\/revisions\/1200"}],"up":[{"embeddable":true,"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/pages\/86"}],"wp:attachment":[{"href":"https:\/\/ri.itservices.manchester.ac.uk\/csf3\/wp-json\/wp\/v2\/media?parent=984"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}